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Issue 12, 2015

An ultrasensitive and selective turn-off fluorescent nanoprobe for the detection of copper ions

Author affiliations

Abstract

In this study, a novel approach for sensitive and extremely selective detection of copper ions has been developed based on fluorescence resonance energy transfer (FRET) between AuNPs as an acceptor and fluorescein isothiocyanate (FITC) as a donor. Initially, the fluorescence of the FITC molecule turns-off due to adsorption to the AuNPs surface. However, the fluorescence of the FITC–AuNPs system was switched to turn-on by adding D-Pencillamine (D-PC) to the solution mixtures as FITC molecules are released upon displacement by D-PC on the NPs surface. The higher affinity of D-PC toward the surface of AuNPs was further evidenced by controlling the fluorescence intensity enhancement of the released FITC in the presence of copper ions. In this scenario, the chelating agent of the D-PC strongly interacts with copper ions, thereby becoming less competitive for adsorption to the AuNPs surface. This methodology was implemented for the determination of copper ions, where under the optimum experimental conditions (pH 8, [FITC] = 1.0 μM, [D-PC] = 6.25 μM), two linear calibration curves were obtained within the range of 1–9 nM and 10–40 nM with a detection limit of 0.3 nM. Ultimately, the designed nanoprobe was successfully employed for the detection of copper ions in food and water samples.

Graphical abstract: An ultrasensitive and selective turn-off fluorescent nanoprobe for the detection of copper ions

Supplementary files

Article information


Submitted
23 Mar 2015
Accepted
06 May 2015
First published
07 May 2015

Anal. Methods, 2015,7, 5067-5073
Article type
Paper
Author version available

An ultrasensitive and selective turn-off fluorescent nanoprobe for the detection of copper ions

M. R. Hormozi-Nezhad and M. Taghipour, Anal. Methods, 2015, 7, 5067 DOI: 10.1039/C5AY00766F

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