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Issue 1, 2015
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A real-time immuno-PCR assay for the detection of tetrabromobisphenol A

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Abstract

In this study, a reliable and ultra-sensitive indirect competitive real-time immuno-PCR (rt-iPCR) was established for the determination of tetrabromobisphenol A (TBBPA). The optimal hapten, artificial immunogen, coating antigen, and polyclonal anti-TBBPA antibodies were successfully prepared. For optimizing rt-iPCR, several physiochemical factors, such as optimal coupling concentration of immunogen and antibody, and the amounts of streptavidin and biotinylated DNA, are discussed. Using this proposed rt-iPCR assay, TBBPA could be determined in the range from 10 pg L−1 to 10 ng L−1, with a detection limit of 2 pg L−1. This rt-iPCR was selective, with low cross-reactivity with TBBPA analogs. Finally, seabed sediment samples were analyzed by rt-iPCR and liquid chromatography. The tested results were demonstrated to be receivable and accurate. The average recovery was between 89.8% and 113.6%. We think this developed rt-iPCR will be very useful in the field of environmental science.

Graphical abstract: A real-time immuno-PCR assay for the detection of tetrabromobisphenol A

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Publication details

The article was received on 05 Jun 2014, accepted on 19 Aug 2014 and first published on 19 Aug 2014


Article type: Paper
DOI: 10.1039/C4AY01343C
Anal. Methods, 2015,7, 99-106

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    A real-time immuno-PCR assay for the detection of tetrabromobisphenol A

    D. Bu, H. Zhuang, G. Yang and X. Ping, Anal. Methods, 2015, 7, 99
    DOI: 10.1039/C4AY01343C

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