Issue 24, 2014

Rapid analysis of essential oils in fruits of Alpinia oxyphylla Miq. by microwave distillation and simultaneous headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry

Abstract

A method based on microwave distillation (MD) and simultaneous headspace solid-phase microextraction (HS-SPME), coupled with gas chromatography-mass spectrometry (GC-MS), was developed for the rapid determination of essential oils in fruits of Alpinia oxyphylla Miq., a traditional Chinese medicine (TCM). HS-SPME conditions, including SPME fiber, desorption time, and microwave parameters (irradiation power and time), were optimized. The method simultaneously accomplishes the isolation, extraction and concentration of the essential oils. Compared to the conventional steam distillation (SD) method, which could only recover 35 compounds, MD-SPME led to the separation and identification of 53 compounds in the essential oils of Alpinia oxyphylla Miq. fruits. The intra-day and inter-day relative standard deviation (RSD) values of the MD-SPME-GC-MS method are all less than 8%, which shows that it has satisfactory precision. The MD-SPME-GC-MS method developed in this study is simple, rapid and solvent-free, and it shows promise for routine analysis of essential oils in fruits of Alpinia oxyphylla Miq. and, potentially, other TCMs.

Graphical abstract: Rapid analysis of essential oils in fruits of Alpinia oxyphylla Miq. by microwave distillation and simultaneous headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry

Article information

Article type
Paper
Submitted
15 Sep 2014
Accepted
12 Oct 2014
First published
28 Oct 2014

Anal. Methods, 2014,6, 9718-9724

Author version available

Rapid analysis of essential oils in fruits of Alpinia oxyphylla Miq. by microwave distillation and simultaneous headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry

D. Wu, K. Chen, M. Xu, G. Song, Y. Hu and H. Cheng, Anal. Methods, 2014, 6, 9718 DOI: 10.1039/C4AY02156H

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