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Issue 9, 2014
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Quantitative-nanoliter immunoassay in capillary immune microreactor adopted inkjet technology

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Abstract

A quantitatively controlled immunoassay at the nanoliter level based on inkjet technology was developed. The volumes of solutions/samples introduced were accurately controlled at the nanoliter level by using a four-channel inkjet microchip. Antibody/antigen recognition was performed in an amino modified capillary with a short diffusion distance. As a proof-of-concept, a sandwich immunoassay of human IgA was conducted using the developed method. The results demonstrated a low detection limit (0.03 ng mL−1) and a wide linear range (0.1–100 ng mL−1, R2 = 0.9959), comparable to currently used methods. For each capillary immunoassay, the volumes of the ejected solutions for human IgA, FITC conjugated anti-human IgA and the glycine–HCl dissociation solution were 52.15 ± 1.53 nL, 65.70 ± 2.06 nL and 37.51 ± 0.96 nL, respectively. This method, in which an inkjet functions as a novel “nanoliter pipette” in combination with a capillary for nanoliter immunoassays, has promising applications in areas of clinical diagnosis and drug screening.

Graphical abstract: Quantitative-nanoliter immunoassay in capillary immune microreactor adopted inkjet technology

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Publication details

The article was received on 24 Jan 2014, accepted on 31 Jan 2014 and first published on 03 Feb 2014


Article type: Communication
DOI: 10.1039/C4AY00216D
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Citation: Anal. Methods, 2014,6, 2832-2836
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    Quantitative-nanoliter immunoassay in capillary immune microreactor adopted inkjet technology

    J. Yang, H. Zeng, S. Xue, F. Chen, H. Nakajima and K. Uchiyama, Anal. Methods, 2014, 6, 2832
    DOI: 10.1039/C4AY00216D

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