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Issue 9, 2014
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Novel validated HPTLC method for estimation of Withaferin A in polyherbal formulations

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Abstract

A novel, accurate, rapid and cost effective quantification method was developed for Withaferin A in herbal combined dosage forms using a destructive reagent. The separation was carried out using mobile phase of toluene : ethyl acetate : formic acid (5 : 5 : 3 v/v/v) at a detection wavelength of 440 nm. The method was validated as per the ICH guideline. The calibration range was found to be 90–630 ng per band. The method was found to be accurate in triplicate results at different standard addition levels. The LOD and LOQ were 2.59 and 7.87 ng respectively. The percentage relative standard deviation was less than 2 for the system suitability testing. The efficiency of the method for separation and quantification was confirmed by applying the same method, without modification, to 11 formulations.

Graphical abstract: Novel validated HPTLC method for estimation of Withaferin A in polyherbal formulations

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Publication details

The article was received on 16 Sep 2013, accepted on 10 Jan 2014 and first published on 13 Jan 2014


Article type: Paper
DOI: 10.1039/C3AY41608A
Anal. Methods, 2014,6, 3009-3012

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    Novel validated HPTLC method for estimation of Withaferin A in polyherbal formulations

    B. H. Patel, J. B. Patel and R. K. Patel, Anal. Methods, 2014, 6, 3009
    DOI: 10.1039/C3AY41608A

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