Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 2, 2014
Previous Article Next Article

Determination of root exudative phenolic compounds in hot pepper (Capsicum annuum L.) by polydimethylsiloxane tube microextraction after derivatization

Author affiliations

Abstract

Polydimethylsiloxane (PDMS) tube microextraction for the determination of 2,3-dimethylphenol (2,3-DMP), 2,6-di-tert-butylphenol (2,6-DTBP) and 4,4′-(1-methylethyliden)bisphenol (4,4′-(1-M)BP) in root exudates of hot pepper (Capsicum annuum L.) by gas chromatography-mass spectrometry (GC-MS) was studied by optimizing derivatization and extraction conditions. Optimum extraction parameters were obtained including PFPy as derivative reagent; derivative reagent volume of 200 μL; sample solution pH of 11; extraction time of 30 min; extraction temperature of 60 °C; isooctane as elution reagent; elution reagent of 1.5 mL. The developed method shows good linearity levels in the range of 0.2–20 μg mL−1 with correlation coefficients of 0.9972, 0.9985 and 0.9978, and relative standard deviations in the range of 4.8–8.4% for 2,3-DMP, 2,6-DTBP and 4,4′-(1-M)BP, respectively, and it's extraction performance was better than that of SPE method. Recoveries ranging from 85.7 to 103.9% and the RSD below 7.9% were obtained when optimized method was applied to determine 2,3-DMP, 2,6-DTBP and 4,4′-(1-M)BP for 15 and 30 days' root hydroponic solution of hot pepper. The developed method is rapid, simple and inexpensive and offers high sensitivity, good reproducibility and satisfactory recovery.

Graphical abstract: Determination of root exudative phenolic compounds in hot pepper (Capsicum annuum L.) by polydimethylsiloxane tube microextraction after derivatization

Back to tab navigation

Article information


Submitted
17 Aug 2013
Accepted
24 Oct 2013
First published
31 Oct 2013

Anal. Methods, 2014,6, 569-574
Article type
Paper

Determination of root exudative phenolic compounds in hot pepper (Capsicum annuum L.) by polydimethylsiloxane tube microextraction after derivatization

H. Sun and Y. Wang, Anal. Methods, 2014, 6, 569
DOI: 10.1039/C3AY41414K

Social activity

Search articles by author

Spotlight

Advertisements