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Issue 23, 2013

Fluorescence spectroscopy and multi-way techniques. PARAFAC

Author affiliations

Abstract

PARAllel FACtor analysis (PARAFAC) is increasingly used to decompose fluorescence excitation emission matrices (EEMs) into their underlying chemical components. In the ideal case where fluorescence conforms to Beers Law, this process can lead to the mathematical identification and quantification of independently varying fluorophores. However, many practical and analytical hurdles stand between EEM datasets and their chemical interpretation. This article provides a tutorial in the practical application of PARAFAC to fluorescence datasets, demonstrated using a dissolved organic matter (DOM) fluorescence dataset. A new toolbox for MATLAB is presented to support improved visualisation and sensitivity analyses of PARAFAC models in fluorescence spectroscopy.

Graphical abstract: Fluorescence spectroscopy and multi-way techniques. PARAFAC

Supplementary files

Article information


Submitted
12 Jul 2013
Accepted
09 Sep 2013
First published
10 Sep 2013

This article is Open Access

Anal. Methods, 2013,5, 6557-6566
Article type
Tutorial Review

Fluorescence spectroscopy and multi-way techniques. PARAFAC

K. R. Murphy, C. A. Stedmon, D. Graeber and R. Bro, Anal. Methods, 2013, 5, 6557 DOI: 10.1039/C3AY41160E

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