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Issue 13, 2013
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Improvement of electrophoretic separation by surface functionalization of a capillary with nano-SiO2 and polyelectrolytes

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Abstract

A bare fused silica capillary lacks enough efficiency for electrophoretic separation under some circumstances as a complex mixture of analogous compounds or stroma matrix. To improve the separation, a strategy of functionalization for the inner surface with nano-silica and polyelectrolytes is proposed in this paper. The results have demonstrated that the hybrid coating film of poly(diallyldimethylammonium chloride) (PDDA)–nano-SiO2–poly(styrenesulfonate) (PSS) on a silica capillary greatly promoted the separation efficiency. As a model, four analytes (naringenin, rutin, quercetin and ascorbic acid) are well separated with 24 mmol L−1 borate buffer solution (pH 8.3) as the carrier. The proposed approach was successfully applied for rapid determination of these analytes in artificial urine with satisfactory results.

Graphical abstract: Improvement of electrophoretic separation by surface functionalization of a capillary with nano-SiO2 and polyelectrolytes

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Article information


Submitted
02 Feb 2013
Accepted
16 Apr 2013
First published
17 Apr 2013

Anal. Methods, 2013,5, 3272-3277
Article type
Paper

Improvement of electrophoretic separation by surface functionalization of a capillary with nano-SiO2 and polyelectrolytes

X. Qian, C. Xiao, Q. Zhang and Y. Tu, Anal. Methods, 2013, 5, 3272
DOI: 10.1039/C3AY40201K

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