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Issue 7, 2013
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Highly selective and water-soluble peptidyl chemosensors for copper(ii) and mercury(ii) ions based on a β-hairpin structure

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Abstract

Based on a β-hairpin structure, two new water-soluble peptidyl chemosensors, HP7-H2H11 and HP7-H1H12, were designed and synthesized. In the peptides we implanted histidine as a metal-binding unit and tryptophan as a reporting unit. These two peptides are sensitive and selective to the detection of Cu2+ and Hg2+ with a detection limit in the range of 0.6 to 1 μM. The dissociation constants (Kd) of Cu2+ binding are smaller than those of Hg2+ binding in HP7-H2H11 and HP7-H1H12, indicating that the peptides have a higher affinity to Cu2+ than Hg2+. pH-dependent fluorescence measurements could be used to discriminate between Cu2+ and Hg2+ in solution due to the fact that these two metal ions had significantly different quenching effects on the fluorescence of HP7-H2H11 and HP7-H1H12 under alkaline conditions. Circular dichroism (CD) measurements further showed that Cu2+ and Hg2+ have distinct effects on the secondary structure of the peptides, providing a complimentary means to differentiate Cu2+ from Hg2+.

Graphical abstract: Highly selective and water-soluble peptidyl chemosensors for copper(ii) and mercury(ii) ions based on a β-hairpin structure

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Supplementary files

Article information


Submitted
10 Dec 2012
Accepted
30 Jan 2013
First published
31 Jan 2013

Anal. Methods, 2013,5, 1702-1707
Article type
Paper

Highly selective and water-soluble peptidyl chemosensors for copper(II) and mercury(II) ions based on a β-hairpin structure

M. Lu, L. Chiu, L. Chiu, C. Lin and J. Horng, Anal. Methods, 2013, 5, 1702
DOI: 10.1039/C3AY26524B

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