Issue 11, 2013
From the journal:

Analytical Methods

A new method for identification of in vitrometabolites of 2,3,7,8-TCDD with rat liver microsomes by using liquid chromatography-mass spectrometry

Chunyan Wang,†ab   Zhi Tang,†a   Yanling Zhang,c   Yan Liang,c   Fengrui Songb  and  Zongwei Cai*a  

* Corresponding authors

a Department of Chemistry, Hong Kong Baptist University, Kowloon, Hong Kong SAR, China
E-mail: zwcai@hkbu.edu.hk
Fax: +852-3411 7348
Tel: +852-3411 7070

b Changchun Institute of Applied Chemistry, Chinese Academic of Sciences, Jilin, China

c Department of Biology, Hong Kong Baptist University, Kowloon, Hong Kong SAR, China

Abstract

2,3,7,8-Tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) is known as a highly toxic environmental contaminant and poses potential risks to human and animal health. As a persistent organic pollutant, 2,3,7,8-TCDD is hard to metabolize and thus gets accumulated in the human and animal body. Literature reports on the metabolism study of 2,3,7,8-TCDD are rare. The traditional method of GC-MS for 2,3,7,8-TCDD analysis might not be amenable for the direct analysis of its polar metabolites. In this study, in vitro metabolism of 2,3,7,8-TCDD with rat liver microsomes by using LC-MS and MS/MS was investigated and two hydroxylated metabolites of 2,3,7,8-TCDD and four trichloro-dihydroxydibenzo-p-dioxins were identified from the direct LC-MS and MS/MS analyses of the incubated samples.

Graphical abstract: A new method for identification of in vitro metabolites of 2,3,7,8-TCDD with rat liver microsomes by using liquid chromatography-mass spectrometry

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Article information

DOI
https://doi.org/10.1039/C3AY26443B
Article type
Paper
Submitted
23 Nov 2012
Accepted
21 Mar 2013
First published
25 Mar 2013

Anal. Methods, 2013,5, 2757-2760

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A new method for identification of in vitro metabolites of 2,3,7,8-TCDD with rat liver microsomes by using liquid chromatography-mass spectrometry

C. Wang, Z. Tang, Y. Zhang, Y. Liang, F. Song and Z. Cai, Anal. Methods, 2013, 5, 2757 DOI: 10.1039/C3AY26443B

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