Issue 4, 2013
From the journal:

Analytical Methods

Direct fluorescence polarization aptamer-based assay for the determination of adenosine

Sheng-E Wangab  and  Shihui Si*a  

* Corresponding authors

a College of Chemistry and Chemical Engineering of Central South University, Changsha, China
E-mail: sishihui@163.com
Fax: +86-73188879616
Tel: +86-73188879616

b Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering of Guangxi Normal University, Guilin, China
E-mail: wse-1978@163.com
Fax: +86 73188879616
Tel: +86 7735854567

Abstract

In the present paper, a direct FP assay strategy for the detection of small size molecules on the basis of a noncompetitive FP technique has been proposed by using adenosine as a small molecule object. The detection limit for adenosine is 26 nM. And this assay has a wide dynamic-range of over five orders of magnitude, from 50 nM to 1.0 mM.

Graphical abstract: Direct fluorescence polarization aptamer-based assay for the determination of adenosine

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Article information

DOI
https://doi.org/10.1039/C2AY26305J
Article type
Communication
Submitted
31 Oct 2012
Accepted
26 Dec 2012
First published
04 Jan 2013

Anal. Methods, 2013,5, 840-843

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Direct fluorescence polarization aptamer-based assay for the determination of adenosine

S. Wang and S. Si, Anal. Methods, 2013, 5, 840 DOI: 10.1039/C2AY26305J

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