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Issue 2, 2013
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Fragmentation of chromophore labelled oligosaccharides induced by photodissociation with visible light

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Abstract

Photodissociation with visible light (Vis-PD) has been applied to chromophore labelled oligosaccharide ions produced by electrospray in a Fourier-transform ion cyclotron resonance (FT-ICR) mass spectrometer. As chromophores four aromatic dyes, such as Rhodamine 110, have been attached to maltopentaose, lacto-N-fucopentaose I (LNFP I) and lacto-N-fucopentaose II (LNFP II). While in the positive ion mode the Vis-PD mass spectra show predominantly glycosidic cleavages and fragments from the reducing end of the carbohydrate, the negative ion spectra result in cross-ring cleavages and also glycosidic fragments mainly from the non-reducing end of the carbohydrate. It will be shown that Vis-PD mass spectrometry leads to sequence specific fragmentation.

Graphical abstract: Fragmentation of chromophore labelled oligosaccharides induced by photodissociation with visible light

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Supplementary files

Article information


Submitted
07 Sep 2012
Accepted
07 Nov 2012
First published
12 Nov 2012

Anal. Methods, 2013,5, 503-507
Article type
Paper

Fragmentation of chromophore labelled oligosaccharides induced by photodissociation with visible light

A. Hahn and J. Grotemeyer, Anal. Methods, 2013, 5, 503
DOI: 10.1039/C2AY26008E

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