Issue 9, 2012
From the journal:

Analytical Methods

The first ratiometric fluorescent probe for aminopeptidase N†

Laizhong Chen,a   Wei Sun,a   Wenhua Li,a   Jing Li,a   Lupei Du,a   Wenfang Xu,a   Hao Fanga  and  Minyong Li*a  

* Corresponding authors

a Department of Medicinal Chemistry, Key Laboratory of Chemical Biology of Natural Products (MOE), School of Pharmacy, Shandong University, Jinan, Shandong 250012, China
E-mail: mli@sdu.edu.cn
Fax: +86-531-8838-2076
Tel: +86-531-8838-2076

Abstract

The current work describes the design and synthesis of the first ratiometric fluorescent probe, Ala-PABA-7HC, for the detection of aminopeptidase N (APN) at both the enzymatic and the cellular level. Upon addition of APN to the solution of Ala-PABA-7HC in PBS or culture medium, the maximum emission peak experienced a red shift from 390 nm to 450 nm, and the ratio of fluorescence intensity (I450/I390) (λex = 330 nm) significantly increased from 0.26 to 3.05 (R = 11.7-fold) in 15 min.

Graphical abstract: The first ratiometric fluorescent probe for aminopeptidase N

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Article information

DOI
https://doi.org/10.1039/C2AY25556A
Article type
Communication
Submitted
30 May 2012
Accepted
22 Jul 2012
First published
23 Jul 2012

Anal. Methods, 2012,4, 2661-2663

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The first ratiometric fluorescent probe for aminopeptidase N

L. Chen, W. Sun, W. Li, J. Li, L. Du, W. Xu, H. Fang and M. Li, Anal. Methods, 2012, 4, 2661 DOI: 10.1039/C2AY25556A

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