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Issue 10, 2012
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Matrix-localization for fast analysis of arrayed microfluidic immunoassays†

Yi Zhang,‡ae   Xuwei Wang,‡b   Lusheng Song,e   Chuanlai Xu,c   Liying Ma,d   Zhanhua Li,b   Jianzhong Xi*a  and  Xingyu Jiang*e  
Author affiliations

* Corresponding authors

a Department of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China
E-mail: xi@coe.pku.edu.cn
Tel: +86-10-62760698

b State Key Laboratory for Nonlinear Mechanics, Institute of Mechanics, Chinese Academy of Sciences, Beijing 100080, China

c School of Food Science and Technology, Jiangnan University, Wuxi 214122, China

d State Key Laboratory for Infection Disease Prevention and Control, National Center for AIDS/STD Control and Prevention (NCAIDS), Chinese Center for Disease Control and Prevention (China-CDC), Beijing 102206, China

e CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing 100190, China
E-mail: xingyujiang@nanoctr.cn
Fax: +86-10-82545631
Tel: +86-10-82545558

Abstract

High-throughput assays necessitate high-throughput data analysis. Arrayed microfluidic immunoassay shows the capability of high-throughput protein detection. However, its development was restricted by the low efficiency of downstream data analysis. We present herein programming-based image processing through the local recognition of a sub-array followed by the region-growing algorithm to achieve fast, convenient and precise extraction of information with reduced personal bias.

Graphical abstract: Matrix-localization for fast analysis of arrayed microfluidic immunoassays

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Article information

https://doi.org/10.1039/C2AY25485A
Submitted
13 May 2012
Accepted
19 Jul 2012
First published
19 Jul 2012
Anal. Methods, 2012,4, 3466-3470
Article type
Technical Note
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Matrix-localization for fast analysis of arrayed microfluidic immunoassays

Y. Zhang, X. Wang, L. Song, C. Xu, L. Ma, Z. Li, J. Xi and X. Jiang, Anal. Methods, 2012, 4, 3466
DOI: 10.1039/C2AY25485A

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