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Issue 4, 2012
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Quantitative estimation of DRDE-07 in mice urine using ion-paired reversed-phase high-performance liquid chromatography

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Abstract

DRDE-07 is a newly synthesized amifostine analog found to be very effective as a prophylactic agent against sulphur mustard toxicity. It has been proven that orally administered DRDE-07 is more efficacious than amifostine, against percutaneously administered SM. We validated an analytical protocol for DRDE-07 in mice urine using high performance liquid chromatographic method. Heptane sulphonic acid and tetramethyl ammonium chloride were used in the mobile phase as an ion pairing agent to perform the chromatographic separation. UV detection was carried out at 249 nm, a wavelength at which an absorption peak was detected. The calibration curve for DRDE-07 was linear in the range from 1 to 100 μg ml−1. The lower limit of quantification (LLOQ) was 1 μg ml−1. The results demonstrate that this method has high linearity (R2 = 0.9986), compound specificity, and acceptable precision/accuracy. The protocol is suitable for in vivo determination of DRDE-07 in mice urine.

Graphical abstract: Quantitative estimation of DRDE-07 in mice urine using ion-paired reversed-phase high-performance liquid chromatography

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Publication details

The article was received on 20 Dec 2011, accepted on 07 Feb 2012 and first published on 09 Mar 2012


Article type: Paper
DOI: 10.1039/C2AY05916A
Anal. Methods, 2012,4, 1019-1023

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    Quantitative estimation of DRDE-07 in mice urine using ion-paired reversed-phase high-performance liquid chromatography

    P. Verma, A. Gautam and R. Vijayaraghavan, Anal. Methods, 2012, 4, 1019
    DOI: 10.1039/C2AY05916A

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