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Issue 1, 2012
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A facile transport assay for H+ coupled membrane transport using fluorescence probes

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Abstract

A facile activity assay for an H+-coupled transporter using florescent probes was developed with an H+-coupled manganese transporter (MntH) as a model. Making use of coupled-proton transport, the transport activity (H+/Mn2+ cotransport) can be directly determined via fluorescence intensity changes of the probe, 5-(and-6)-carboxyfluorescein (5(6)-FAM). The approach of using highly sensitive fluorescence probes provides a more simple and convenient assay method for the determination of proton-coupled metal-ion uptake by transporters.

Graphical abstract: A facile transport assay for H+ coupled membrane transport using fluorescence probes

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Publication details

The article was received on 02 Sep 2011, accepted on 02 Nov 2011 and first published on 14 Nov 2011


Article type: Communication
DOI: 10.1039/C1AY05549F
Citation: Anal. Methods, 2012,4, 44-46
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    A facile transport assay for H+ coupled membrane transport using fluorescence probes

    W. Lan, H. Ren, Y. Pang, C. Huang, Y. Xu, R. J. Brooker and J. Zhang, Anal. Methods, 2012, 4, 44
    DOI: 10.1039/C1AY05549F

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