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Issue 1, 2012
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A specific UPLC-ESI-MS/MS method for analysis of cyadox and its three main metabolites in fish samples

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Abstract

A rapid and sensitive ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed for the determination of cyadox (CYX) and its three major metabolites, quinoxaline-2-carboxylic acid (QCA), 1-desoxycyadox (1-DCYX) and 1,4-bisdesoxycyadox (BDCYX) in fish. Samples were extracted with acetonitrile–0.5 M hydrochloric acid (9 + 1) and cleaned up using Oasis MAX cartridges. The total time taken for separation by UPLC was less than 5 min. The four target compounds were then determined by ESI-MS/MS. At the fortified levels of 2–50 μg kg−1 in fish, recoveries of all the compounds ranged from 80.2% to 88.2%, with a relative standard deviation of 6.8 to 14.6%. Limits of detection for CYX, QCA, 1–DCYX and BDCYX were 0.52, 1.07, 0.69 and 0.38 μg kg−1, respectively.

Graphical abstract: A specific UPLC-ESI-MS/MS method for analysis of cyadox and its three main metabolites in fish samples

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Publication details

The article was received on 22 Aug 2011, accepted on 12 Nov 2011 and first published on 15 Dec 2011


Article type: Paper
DOI: 10.1039/C1AY05523B
Anal. Methods, 2012,4, 217-221

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    A specific UPLC-ESI-MS/MS method for analysis of cyadox and its three main metabolites in fish samples

    L. Cheng, Z. Wang, J. Shen, L. Li, H. Wu and S. Zhang, Anal. Methods, 2012, 4, 217
    DOI: 10.1039/C1AY05523B

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