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Issue 6, 2012
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Determination of anticonvulsants in human plasma using SPME in a heated interface coupled online to liquid chromatography (SPME-LC)

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Abstract

A simple and sensitive method using solid phase microextraction (SPME) and liquid chromatography (LC) with heated online desorption (SPME-LC) was developed and validated to analyze anticonvulsants (AEDs) in human plasma samples. A heated lab-made interface chamber was used in the desorption procedure, which allowed the transference of the whole extracted sample. The SPME conditions were optimized by applying an experimental design. Important factors are discussed such as fiber coating types, pH, extraction time and desorption conditions. The drugs were analyzed by LC, using a C18 column (150 mm × 4.6 mm × 5 μm); and 50 mmol L−1, pH = 5.50 ammonium acetate buffer : acetonitrile : methanol (55 : 22 : 23 v/v) as the mobile phase with a flow rate of 0.8 mL min−1. The suggested method presented precision (intra-assay and inter-assay), linearity and limit of quantification (LOQ) all adequate for the therapeutic drug monitoring (TDM) of AEDs in plasma.

Graphical abstract: Determination of anticonvulsants in human plasma using SPME in a heated interface coupled online to liquid chromatography (SPME-LC)

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Article information


Submitted
13 Jan 2012
Accepted
16 Apr 2012
First published
17 Apr 2012

Anal. Methods, 2012,4, 1519-1524
Article type
Paper

Determination of anticonvulsants in human plasma using SPME in a heated interface coupled online to liquid chromatography (SPME-LC)

C. Alves, P. C. F. de Lima Gomes, Á. J. dos Santos-Neto, J. C. Rodrigues and F. M. Lanças, Anal. Methods, 2012, 4, 1519
DOI: 10.1039/C2AY25047K

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