Jump to main content
Jump to site search

Issue 12, 2011
Previous Article Next Article

PARAFAC based methods for the analysis of Diltiazemdrug excitation emission matrices of fluorescence obtained by a derivatization reaction

Author affiliations

Abstract

A fluorescent derivatization reaction for Diltiazem drug quantification based on the condensation reaction of citric or malonic acid with acetic anhydride, catalyzed by the tertiary amine group of Diltiazem, was developed. Excitation emission matrices (EEMs) of fluorescence of the pure solvent (ethanol), standard and sample solutions following a standard addition methodology were analysed by PARAFAC based methods (PARAFAC, PARAFAC2 and PARALIND) to obtain robust calibration methodologies. The quantification results of the sample were compared with the official US Pharmacopeia high performance liquid chromatography-ultraviolet method (USP HPLC-UV). Although the experimental sets of EEM show linearity deviations all the PARAFAC based methods allow correct robust estimation of Diltiazem concentration in pharmaceutical formulations. The closest results were: derivatization with citric acid and PARAFAC2 six components non-negativity constraint model with a detection limit of 0.088 ppm; and, derivatization with malonic acid and PARAFAC six components non-negativity constraint model with a detection limit of 0.066 ppm for the malonic acid was observed. The simultaneous utilization of the three PARAFAC methods gives further information about the intrinsic structure of the data sets under analysis, i.e., it works as an efficient diagnostic tool for the existence of non-linearity and colinearity.

Graphical abstract: PARAFAC based methods for the analysis of Diltiazem drug excitation emission matrices of fluorescence obtained by a derivatization reaction

Back to tab navigation

Publication details

The article was received on 05 Sep 2011, accepted on 05 Oct 2011 and first published on 17 Nov 2011


Article type: Paper
DOI: 10.1039/C1AY05560G
Anal. Methods, 2011,3, 2758-2769

  •   Request permissions

    PARAFAC based methods for the analysis of Diltiazem drug excitation emission matrices of fluorescence obtained by a derivatization reaction

    J. M. M. Leitão, E. F. C. Simões and J. C. G. Esteves da Silva, Anal. Methods, 2011, 3, 2758
    DOI: 10.1039/C1AY05560G

Search articles by author

Spotlight

Advertisements