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Issue 10, 2011
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Application of bimetallic nanoparticles modified screen printed electrode for the detection of organophosphate compounds using an enzyme inhibition approach

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Abstract

A novel, unique goldplatinum bimetallic nanoparticles modified screen printed electrode (SPE) has been developed for the sensitive detection of paraoxon ethyl, carbofuran and simulant of nerve agent diisopropyl fluorophosphate (DFP), based on the enzyme inhibition approach. The goldplatinum nanoparticles were electrochemically deposited on a 3-aminopropyl triethoxy silane modified electrode. Following this acetylcholineesterase (AChE)/choline oxidase (ChOx) bienzymes were immobilized through cross-linking with glutaraldehyde onto a modified electrode. Electrodes were characterized by scanning electron microscopy (SEM), EDX (energy dispersive X-ray analysis) and cyclic voltammetry (CV). The bimetallic alloy nanoparticles have an excellent high surface area and the unique effect of electrocatalytic activity for the oxidation of hydrogen peroxide (H2O2). The IC50 values were determined for the inhibitors using immobilized enzymes on the modified electrode. Paraoxon ethyl, carbofuran, and DFP could be detected up to 0.20 μM, 0.20–0.25 μM, and 0.20–0.25 μM respectively at a 25–30% inhibition level of AChE enzyme (residual enzyme activity) with an incubation time of 10 min.

Graphical abstract: Application of bimetallic nanoparticles modified screen printed electrode for the detection of organophosphate compounds using an enzyme inhibition approach

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Supplementary files

Article information


Submitted
29 Apr 2011
Accepted
29 Jun 2011
First published
23 Aug 2011

Anal. Methods, 2011,3, 2246-2253
Article type
Paper

Application of bimetallic nanoparticles modified screen printed electrode for the detection of organophosphate compounds using an enzyme inhibition approach

S. Upadhyay, M. K. Sharma, G. Rama Rao, B. K. Bhattacharya, V. K. Rao and R. Vijayaraghavan, Anal. Methods, 2011, 3, 2246
DOI: 10.1039/C1AY05252G

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