A resonance light scattering amplification system for determination of trace amounts of benzidine in surface water

Abstract

In this contribution, a novel resonance light scattering (RLS) amplification system was developed based on the enhanced RLS signals of the ammonium molybdate (AM)–cetyl trimethyl ammonium bromide (CTMAB) system upon the addition of benzidine (BD). It was found that in the presence of CTMAB, trace amounts of BD in surface water induced a remarkable change in RLS intensity of the AM–CTMAB system. Moreover, in the range of 0.005–1.0 μg mL⁻¹, there was a good linear relationship between the enhanced RLS intensity and the concentration of BD with a detection limit of 0.08 ng mL⁻¹. Three kinds of surface water sample were determined by this assay, and the interaction mechanism between AM and BD as well as several influencing factors were also investigated. Compared with conventional assays for the determination of BD in water, this assay has the advantages of simplicity, high sensitivity and it is more suitable for the determination of trace amounts of micromolecules.