Jump to main content
Jump to site search

Issue 9, 2011
Previous Article Next Article

Simple method for simultaneous detection of uric acid, xanthine and hypoxanthine in fish samples using a glassy carbon electrode modified with as commercially received multiwalled carbon nanotubes

Author affiliations

Abstract

A simple electrochemical sensor was designed, based on a glassy carbon electrode modified with “as commercially received” multiwalled carbon nanotubes (GCE/MWCNT), for the simultaneous detection of three purine bases: uric acid (UA), xanthine (X) and hypoxanthine (Hx) by differential pulse voltammetry (DPV). Comparison amongst various carbon nanotubesviz.; as commercially received-MWCNT, functionalized-MWCNT, purified-MWCNT and single walled carbon nanotubes for modified electrode preparation, and in turn for the simultaneous electrochemical detection of the purines by DPV, the “as received MWCNT” modified GCE showed the best performance in terms of well-separated peaks and higher peak current values. Scan rate experiments on discreet oxidations of UA, X and Hx suggested that the electron-transfer mechanism for both UA and X follows a mixed diffusion–adsorption controlled pathway, while in the case of Hx, a diffusion controlled route is followed. Calibration responses for the simultaneous detection of UA, X and Hx were linear up to 700, 200 and 150 μM respectively. Corresponding detection limit values were 141 nM, 134 nM and 2.87 μM. Finally analysis of UA, X and Hx content in three different fresh dead fish samples was successfully demonstrated with recoveries of around 100%.

Graphical abstract: Simple method for simultaneous detection of uric acid, xanthine and hypoxanthine in fish samples using a glassy carbon electrode modified with as commercially received multiwalled carbon nanotubes

Back to tab navigation

Supplementary files

Publication details

The article was received on 07 Feb 2011, accepted on 05 Jul 2011 and first published on 11 Aug 2011


Article type: Paper
DOI: 10.1039/C1AY05065F
Anal. Methods, 2011,3, 2088-2094

  •   Request permissions

    Simple method for simultaneous detection of uric acid, xanthine and hypoxanthine in fish samples using a glassy carbon electrode modified with as commercially received multiwalled carbon nanotubes

    A. S. Kumar and R. Shanmugam, Anal. Methods, 2011, 3, 2088
    DOI: 10.1039/C1AY05065F

Search articles by author

Spotlight

Advertisements