Issue 7, 2011
From the journal:

Analytical Methods

A new DNA extraction method for automated food analysis

Alexander W. Gruentzig,ab   Catherine M. Klapperich,cd   Andre Sharon,ad   Jeff Braman,e   Anirban Chatterjeead  and  Alexis F. Sauer-Budge*ac  

* Corresponding authors

a Fraunhofer USA, Center for Manufacturing Innovation, Brookline, MA, USA
E-mail: asauerbudge@fraunhofer.org

b Division of Materials Science and Engineering, Boston University, Brookline, MA, USA

c Department of Biomedical Engineering, Boston University, Boston, MA, USA

d Department of Mechanical Engineering, Boston University, Boston, MA, USA

e Agilent Technologies, Stratagene Products Division, La Jolla, CA, USA

Abstract

Effective DNA purification is essential for reliable composition and contamination analysis in the food industry. This paper compares two DNA extraction and purification procedures for food analysis. The commercially available DNA extraction kit from Agilent Technologies, Inc. was tested against a new solid phase extraction (SPE) method using silica beads embedded in a porous monolith structure that is integrated in a disposable column design. The technique is amenable to automation and streamlined workflow integration, with possible miniaturization of the sample handling process making it suitable for high-throughput applications. To demonstrate the automated process, the manual homogenizing step was compared to a custom automated procedure. To demonstrate the process for food testing, two types of sausages were analyzed for traces of porcine gDNA.

Graphical abstract: A new DNA extraction method for automated food analysis

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Article information

DOI
https://doi.org/10.1039/C0AY00701C
Article type
Paper
Submitted
18 Nov 2010
Accepted
21 Apr 2011
First published
02 Jun 2011

Anal. Methods, 2011,3, 1507-1513

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A new DNA extraction method for automated food analysis

A. W. Gruentzig, C. M. Klapperich, A. Sharon, J. Braman, A. Chatterjee and A. F. Sauer-Budge, Anal. Methods, 2011, 3, 1507 DOI: 10.1039/C0AY00701C

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