A flow injection electrochemiluminescence (FI-ECL) analysis method for the determination of L-lysine, in alkaline Na2CO3-NaHCO3 buffer solution, in the presence of tris(2,2′-bipyridyl) ruthenium(II) (Ru(bpy)32+) on indium tin oxide (ITO) glass was studied by conventional cyclic voltammetry. This method is based on the enhanced ECL of Ru(bpy)32+- L-lysine. Meanwhile, in order to overcome the drawbacks of conventional cells, a new ECL cell was designed. Under the optimal conditions, the enhanced ECL intensity was linearly related to the concentration of L-lysine in the range from 1.0 × 10−8 to 1.0 × 10−4 g mL−1 with a detection limit 1.43 × 10−9 g mL−1. The relative standard deviation (RSD) for the determination of 1.0 × 10−6 g mL−1L-lysine was 1.8% (n = 11). The possible mechanism is discussed. In this paper, the ECL behavior of Ru(bpy)32+ is studied on ITO glass, and the newly designed sensor is reusable, shows a great improvement in sensitivity and selectivity for ECL analysis, and can be successfully applied for amino acid analysis.
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