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Issue 10, 2010
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Studying copper(ii) ion induced interactions of β-amyloidpeptides within living cells by gold nanoparticleprobes

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Abstract

In this paper, a kind of gold nanoparticle (GNP)-based colorimetric assay was developed for studying copper(II) ion (Cu2+) induced interaction of β-amyloid (Aβ) peptide with living cells. In the presence of Cu2+, biotin modified β-amyloid peptides (116(biotin)) enable to attach on the SHG-44 (human glioma cell) cellular surface which results in significant color change of the cells when the indicators, streptavidin conjugated GNPs were introduced into the reaction mixture. We also demonstrate the assay ability to screen Aβ aggregation inhibitors because the aggregating/binding process can be easily reversed by adding chelators (e.g., EDTA, EGTA, histidine and clioquinol) of Cu2+ to the system. In addition, a time-dependent interaction of SHG-44 cells with Cu2+ and 116(biotin) has been observed that may suggest different expression levels of Aβ related proteins in various cell cycles.

Graphical abstract: Studying copper(ii) ion induced interactions of β-amyloid peptides within living cells by gold nanoparticle probes

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Publication details

The article was received on 09 Jul 2010, accepted on 27 Jul 2010 and first published on 09 Sep 2010


Article type: Paper
DOI: 10.1039/C0AY00438C
Anal. Methods, 2010,2, 1467-1471

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    Studying copper(II) ion induced interactions of β-amyloid peptides within living cells by gold nanoparticle probes

    C. Wang, J. Wang, D. Liu and Z. Wang, Anal. Methods, 2010, 2, 1467
    DOI: 10.1039/C0AY00438C

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