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Issue 12, 2010
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Progress curve analysis of enzyme reactions by flow microcalorimetry: Use of a pseudo-first order approximation

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Abstract

We describe a method for determining the pseudo-first order rate constant of a chemical reaction by flow microcalorimetry operating in the flow-through mode. The impulse response of the instrument was described by a Gamma distribution and the equation of the thermogram was computed analytically. The resulting equation was fitted to the data by simulated annealing. The method was applied to an enzyme reaction following Michaelis–Menten kinetics by means of a new empirical equation relating the apparent rate constant to the kinetic parameters Vmax and Km. The method was exemplified by a kinetic study of horse serum butyrylcholinesterase.

Graphical abstract: Progress curve analysis of enzyme reactions by flow microcalorimetry: Use of a pseudo-first order approximation

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Publication details

The article was received on 04 Apr 2010, accepted on 13 Sep 2010 and first published on 08 Oct 2010


Article type: Paper
DOI: 10.1039/C0AY00223B
Anal. Methods, 2010,2, 1958-1961

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    Progress curve analysis of enzyme reactions by flow microcalorimetry: Use of a pseudo-first order approximation

    J. Debord, M. Harel, J. Bollinger and T. Dantoine, Anal. Methods, 2010, 2, 1958
    DOI: 10.1039/C0AY00223B

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