Jump to main content
Jump to site search

Issue 5, 2021
Previous Article Next Article

Solid-state colorimetric polydiacetylene liposome biosensor sensitized by gold nanoparticles

Author affiliations

Abstract

Polydiacetylene (PDA), a conjugated polymer, has attracted attention for realization of a label-free real-time colorimetric biosensor because it exhibits large and rapid colorimetric responses upon the binding of biomolecules. This is due to the conformational distortion of its conjugated backbone. However, solid-state PDA biosensors for point-of-care diagnosis remain unexplored. We describe a highly sensitive solid-state biosensor based on PDA liposomes. We employed gold nanoparticles (AuNPs) on PDA liposomes as the molecular-binding-signal sensitizer, which provides additional conformational distortion in the backbone structure of PDA by exerting steric repulsion to the attached biomolecules. To prove the concept, AuNPs and a thrombin-binding-aptamer were individually functionalized on PDA liposomes, which were attached to a substrate for the detection of thrombin. We found that the sensitivity was enhanced 2.5 times in the presence of AuNPs compared with the case without AuNPs. Because the steric repulsion of the AuNPs is target-independent, we believe that our solid-state biosensor provides a path toward advanced solid-state biosensors.

Graphical abstract: Solid-state colorimetric polydiacetylene liposome biosensor sensitized by gold nanoparticles

Back to tab navigation

Article information


Submitted
11 Dec 2020
Accepted
02 Jan 2021
First published
15 Jan 2021

Analyst, 2021,146, 1682-1688
Article type
Paper

Solid-state colorimetric polydiacetylene liposome biosensor sensitized by gold nanoparticles

J. Kim, B. Moon, E. Hwang, S. Shaban, W. Lee, D. Pyun, D. H. Lee and D. Kim, Analyst, 2021, 146, 1682
DOI: 10.1039/D0AN02375B

Social activity

Search articles by author

Spotlight

Advertisements