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Issue 4, 2021

Label-free analysis of gingival crevicular fluid (GCF) by surface enhanced Raman scattering (SERS)

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Abstract

Gingival crevicular fluid (GCF) is an interesting biofluid reflecting the physiological and pathological states of a single dental element. Due to this unique feature, in recent years, metabolomic analysis of GCF has gained attention as a biometric tool for the diagnosis and therapy of periodontal disease. Traditional methods are, however, too slow, cumbersome and expensive for a health-care routine. Surface enhanced Raman scattering (SERS) can offer rapid and label-free detailed molecular fingerprints that can be used for biofluid analysis. Here we report the first SERS characterization of GCF using an easy and quick sample preparation. The dominant features in the SERS spectrum of GCF are ascribed to very few metabolites, in particular to uric acid, hypoxanthine, glutathione and ergothioneine. Additionally, we succeeded in differentiating between the SERS signal of GCF collected from healthy volunteers and the one collected from patients with periodontal disease.

Graphical abstract: Label-free analysis of gingival crevicular fluid (GCF) by surface enhanced Raman scattering (SERS)

Supplementary files

Article information


Submitted
09 Oct 2020
Accepted
15 Dec 2020
First published
16 Dec 2020

Analyst, 2021,146, 1464-1471
Article type
Paper

Label-free analysis of gingival crevicular fluid (GCF) by surface enhanced Raman scattering (SERS)

S. Fornasaro, F. Berton, C. Stacchi, F. Farina, A. Esposito, V. Sergo, R. Di Lenarda and A. Bonifacio, Analyst, 2021, 146, 1464 DOI: 10.1039/D0AN01997F

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