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Issue 21, 2020
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Thread-based isoelectric focusing coupled with desorption electrospray ionization mass spectrometry

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Abstract

The combination of a thread-based electrofluidic analytical device and desorption electrospray ionization mass-spectrometry (DESI-MS) was investigated for the separation and concentration of proteins. The combination delivered a low-cost novel approach for sample pretreatment and target focusing, with direct “on-thread” ambient mass spectrometry detection. For this purpose, a platform for thread-based isoelectric focusing (TB-IEF) was 3D-printed, optimised, and applied to the separation and focusing of three model proteins. Successful separation and focusing was achieved within 30 min. The TB-IEF device was coupled with DESI-MS by direct exposure of the focused solutes on the dried thread to the DESI source. As a proof-of-concept, a 10-fold increase in the DESI-MS response for insulin was achieved following the TB-IEF preconcentration, whilst simultaneously isolating the target solutes from their sample matrix.

Graphical abstract: Thread-based isoelectric focusing coupled with desorption electrospray ionization mass spectrometry

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Supplementary files

Article information


Submitted
06 Jul 2020
Accepted
18 Aug 2020
First published
18 Aug 2020

Analyst, 2020,145, 6928-6936
Article type
Paper

Thread-based isoelectric focusing coupled with desorption electrospray ionization mass spectrometry

L. Chen, J. M. Cabot, E. Sanz Rodriguez, A. Ghiasvand, P. C. Innis and B. Paull, Analyst, 2020, 145, 6928
DOI: 10.1039/D0AN01344G

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