A polypyrrole-coated eightfold-helical Wells–Dawson POM-based Cu-FKZ framework for enhanced colorimetric sensing

Abstract

To explore a novel colorimetric biosensor with high sensibility and selectivity, a new Wells–Dawson-type polyoxometalate (POM)-based metal–organic framework (MOF) with an eightfold helix, [Cu₉(FKZ)₁₂(H₂O)₈][H₃P₂W₁₈O₆₂]₂·4H₂O (CuFKZP₂W₁₈) (HFKZ = 1-(2,4-difluorophenyl)-1,1-bis[(1H-1,2,4-triazol-1-yl)methyl] ethanol), was successfully synthesized; then, polypyrrole (PPy) was introduced to fabricate CuFKZP₂W₁₈/PPy(n) nanocomposites (n = 7%, 15%, 30%) via a facile in situ oxidation polymerization process. All the results indicate that CuFKZP₂W₁₈/PPy(15%) as a colorimetric biosensor exhibits lower limits of detection (0.07 μM towards H₂O₂ and 0.627 μM towards ascorbic acid), smaller K_m values (0.106 mM for H₂O₂ and 0.042 mM for o-phenylenediamine) and higher sensitivity (0.0227 1 μM⁻¹ towards H₂O₂ and 0.0025 1 μM⁻¹ to ascorbic acid) than most reported enzyme mimetics to the best of our knowledge.