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Issue 6, 2020
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A gold nanoparticle-based lateral flow immunosensor for ultrasensitive detection of tetrodotoxin

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Abstract

Tetrodotoxin (TTX) is a potent marine neurotoxin. Its frequent detection and widespread distribution pose a substantial health risk to consumers. The work presented herein describes the development of a lateral flow immunosensor based on a gold nanoparticle-labeled monoclonal antibody probe, which allows the affinity reaction of antigen–antibody and amplifies the reaction signal. The immunosensor assay was found to provide a visible limit of detection (vLOD) of 10 μg kg−1 in crucian and clam matrices without time-consuming pretreatment. Spiked samples were analyzed using the immunosensor and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The immunosensor gave average intra-assay recoveries of 84.0–103.9%, inter-assay recoveries of 76.1–105% for the crucian matrix and intra-assay recoveries of 78.5–93.0%, inter-assay recoveries of 74.8–85.7% for the clam matrix. The results obtained using the immunosensor and LC-MS/MS were in good agreement. The immunosensor test could be completed within 10 min, offering a convenient option or complementarity to the traditional methods of TTX detection.

Graphical abstract: A gold nanoparticle-based lateral flow immunosensor for ultrasensitive detection of tetrodotoxin

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Supplementary files

Article information


Submitted
22 Jan 2020
Accepted
10 Feb 2020
First published
12 Feb 2020

Analyst, 2020,145, 2143-2151
Article type
Paper

A gold nanoparticle-based lateral flow immunosensor for ultrasensitive detection of tetrodotoxin

Y. Li, X. Xu, L. Liu, H. Kuang, L. Xu and C. Xu, Analyst, 2020, 145, 2143
DOI: 10.1039/D0AN00170H

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