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Issue 4, 2020
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A high-selectivity fluorescent probe for hypoxia imaging in cells and a tumor-bearing mouse model

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Abstract

Nitroreductase (NTR) with a high expression level in tumors has been considered as a biomarker of highly aggressive hypoxia tumors. Thus, it is important to develop powerful tools for tumor hypoxia detection. Here, we developed a two-photon fluorescent probe hTP-NNO2 for NTR detection. The probe with one-step synthesis exhibited high yield. hTP-NNO2 showed high selectivity and sensitivity for NTR and the detection limit was as low as 43 ng mL−1. hTP-NNO2 also showed low cytotoxicity and high stability, indicating that hTP-NNO2 is suitable for NTR detection in real-time and in situ under physiological conditions. hTP-NNO2 was used for NTR imaging in hypoxia cells and the fluorescence intensity of hTP-NNO2 increased with decreasing oxygen concentration. Benefiting from the advantages of two-photon fluorescent probes, we performed NTR detection in deep brain tissue with an imaging depth of up to 100 μm. hTP-NNO2 was further successfully applied for NTR detection in zebrafish and tumors. These results indicated that we developed a promising fluorescence imaging tool for NTR detection in vitro and in vivo.

Graphical abstract: A high-selectivity fluorescent probe for hypoxia imaging in cells and a tumor-bearing mouse model

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Supplementary files

Article information


Submitted
03 Dec 2019
Accepted
06 Jan 2020
First published
07 Jan 2020

Analyst, 2020,145, 1389-1395
Article type
Paper

A high-selectivity fluorescent probe for hypoxia imaging in cells and a tumor-bearing mouse model

Y. Wang, X. Han, X. Zhang, L. Zhang and L. Chen, Analyst, 2020, 145, 1389 DOI: 10.1039/C9AN02436K

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