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Issue 4, 2020
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Branch migration-based polymerase chain reaction combined with endonuclease IV-assisted target recycling probe/blocker system for detection of low-abundance point mutations

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Abstract

Sensitive detection of low-abundance point mutations in blood or tissue may provide a great opportunity for the minimally invasive diagnosis of cancer and other related diseases. We demonstrate a novel method for ultra-sensitive detection of point mutations at low abundance by combination of branch migration-based PCR with endonuclease IV-assisted target recycling probe/blocker system. The method is able to identify the point mutations at abundances down to 0.01–0.02%. We anticipate this method to be widely adopted in clinical diagnosis and molecular research.

Graphical abstract: Branch migration-based polymerase chain reaction combined with endonuclease IV-assisted target recycling probe/blocker system for detection of low-abundance point mutations

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Article information


Submitted
04 Nov 2019
Accepted
15 Jan 2020
First published
16 Jan 2020

Analyst, 2020,145, 1355-1361
Article type
Paper

Branch migration-based polymerase chain reaction combined with endonuclease IV-assisted target recycling probe/blocker system for detection of low-abundance point mutations

N. Chen, W. Zhang, X. Tang, Z. Ming and X. Xiao, Analyst, 2020, 145, 1355 DOI: 10.1039/C9AN02209K

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