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Issue 21, 2019
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A new colorimetric assay method for the detection of anti-hepatitis C virus antibody with high sensitivity

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Abstract

The early detection of low abundance anti-hepatitis C virus antibody (anti-HCV Ab) is critical for efficient diagnosis and treatment of HCV infection. In this work, a new colorimetric assay method has been proposed for the sensitive detection of anti-HCV Ab. In this method, the antibody-induced DNA strand displacement and the resulting rolling circle amplification (RCA) are integrated to generate a large amount of tandemly repeated G-quadruplex DNAzymes on the arm of the “Y”-shaped antibody. Consequently, oxidation of 3,3′,5,5′-tetramethylbenzidine can be extensively catalyzed by the peroxidase-mimicking DNAzymes. Therefore, the readout signal can be greatly amplified. Further studies reveal that 0.998 pM anti-HCV Ab can be detected by this newly developed assay method. Moreover, the strategy proposed in this method can be adapted for the detection of other antibodies or bivalent targets.

Graphical abstract: A new colorimetric assay method for the detection of anti-hepatitis C virus antibody with high sensitivity

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Publication details

The article was received on 02 Aug 2019, accepted on 04 Sep 2019 and first published on 05 Sep 2019


Article type: Paper
DOI: 10.1039/C9AN01466G
Analyst, 2019,144, 6365-6370

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    A new colorimetric assay method for the detection of anti-hepatitis C virus antibody with high sensitivity

    T. Gao, W. Chai, L. Shi, H. Shi, A. Sheng, J. Yang and G. Li, Analyst, 2019, 144, 6365
    DOI: 10.1039/C9AN01466G

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