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Issue 10, 2019
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Monitoring mitochondrial pH with a hemicyanine-based ratiometric fluorescent probe

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Abstract

Mitochondria as essential organelles play critical roles in cellular metabolism. Mitochondrial pH is a vital parameter that directly affects the unique function of mitochondria. Herein, we present a new ratiometric fluorescent probe M-pH for monitoring the pH within the mitochondria. M-pH consists of a stable and large π-electron conjugated merocyanine system. The lipophilic cationic benzyl group will facilitate the accumulation of M-pH in mitochondria. The phenol unit is the recognition moiety, achieving the ratiometric sensing of pH changes. The experimental results indicate that M-pH displays ratiometric fluorescence response to different pH values. Meanwhile, M-pH shows negligible response to common species, and has high stability and low cytotoxicity. In biological experiments, M-pH can solely accumulate in mitochondria and visualize the pH changes during mitophagy and cell apoptosis. We thus believe that M-pH has great potential as a practical tool for real-time monitoring of pH changes of mitochondria, contributing to revealing the pathogenesis of mitochondrial pH associated diseases.

Graphical abstract: Monitoring mitochondrial pH with a hemicyanine-based ratiometric fluorescent probe

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Supplementary files

Article information


Submitted
05 Mar 2019
Accepted
11 Apr 2019
First published
12 Apr 2019

Analyst, 2019,144, 3422-3427
Article type
Paper

Monitoring mitochondrial pH with a hemicyanine-based ratiometric fluorescent probe

H. Xiao, Y. Dong, J. Zhou, Z. Zhou, X. Wu, R. Wang, Z. Miao, Y. Liu and S. Zhuo, Analyst, 2019, 144, 3422
DOI: 10.1039/C9AN00422J

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