Jump to main content
Jump to site search

Issue 10, 2019
Previous Article Next Article

A resorufin-based fluorescent probe for imaging polysulfides in living cells

Author affiliations

Abstract

As the oxidative metabolites of the endogenous gasotransmitter H2S, H2Sn have attracted ever-increasing attention in the field of biomedical research due to their vital functions in biological systems. Herein, we report a resorufin-based “turn-on” probe for H2Sn sensing. An SNAr substitution–intramolecular cyclization cascade reaction was used in this probe for detecting exogenous and endogenous H2Sn. The detection process could be monitored using UV-Vis and fluorescence spectroscopy and the naked eye. The emission response of the probe towards H2Sn presented a good linear relationship in the 0–50 μM concentration range, and the LOD of this probe was 24 nM. The probe was used successfully to visualize endogenous H2Sn generated in the RAW 246.7 cells under external stimulation.

Graphical abstract: A resorufin-based fluorescent probe for imaging polysulfides in living cells

Back to tab navigation

Supplementary files

Publication details

The article was received on 26 Feb 2019, accepted on 05 Apr 2019 and first published on 23 Apr 2019


Article type: Communication
DOI: 10.1039/C9AN00377K
Analyst, 2019,144, 3221-3225

  •   Request permissions

    A resorufin-based fluorescent probe for imaging polysulfides in living cells

    J. Liu and Z. Yin, Analyst, 2019, 144, 3221
    DOI: 10.1039/C9AN00377K

Search articles by author

Spotlight

Advertisements