Jump to main content
Jump to site search

Issue 14, 2019
Previous Article Next Article

Exploiting the application of l-aptamer with excellent stability: an efficient sensing platform for malachite green in fish samples

Author affiliations

Abstract

Effective monitoring of the content of malachite green (MG) in aquaculture is of great importance for food safety. Traditional methods for MG assay, such as chromatography and spectroscopy, have been criticized for expensive instrumentation and complicated pretreatments. An MG RNA aptamer (MGA) is a powerful tool for immediate and rapid detection of MG. However, RNA is easily degraded by nucleases and is unstable in the environment, making accurate and reliable detection of MG difficult. In order to address the problems, an innovative levo (L)-MGA with excellent stability is designed to perform the specific recognition function. Interestingly, the gel electrophoresis and fluorescence measurement results indicate that this unnaturally occurring L-aptamer is resistant to nuclease degradation and it can be kept intact in the standard buffer solution under room temperature for quite a long time. A label-free, simple, and efficient method has been developed for sensitive detection of MG in fish tissue, which holds promising potential in food analysis and environmental monitoring.

Graphical abstract: Exploiting the application of l-aptamer with excellent stability: an efficient sensing platform for malachite green in fish samples

Back to tab navigation

Article information


Submitted
18 Feb 2019
Accepted
15 May 2019
First published
23 May 2019

Analyst, 2019,144, 4204-4209
Article type
Paper

Exploiting the application of L-aptamer with excellent stability: an efficient sensing platform for malachite green in fish samples

X. Luo, Z. Chen, H. Li, W. Li, L. Cui and J. Huang, Analyst, 2019, 144, 4204 DOI: 10.1039/C9AN00332K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements