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Issue 13, 2019
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Ultrasensitive detection of H. pylori in human feces based on immunomagnetic bead capture and fluorescent quantum dots

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Abstract

Given that Helicobacter pylori (H. pylori) generally infects people in early childhood and that such persons when not treated with antibiotics remain infected for the rest of their lives, it is quite important to detect H. pylori in children, and convenient to do so using non-invasive methods. Stool antigen tests constitute such an effective non-invasive method. In the current work, a novel fecal test was developed to detect H. pylori based on immunomagnetic beads (IMBs) with monoclonal antibodies sensitively recognizing and capturing the H. pylori, coupled with a polyclonal antibody-conjugating quantum dot probe, and ultrasensitive detection was achieved by using a fluorescence spectrometer. The detection method took 120 min to perform, and showed a limit of detection of 102 CFU mL−1 and a linear range of 10 to 106 CFU mL−1 (R2 = 0.9962). Most importantly, this method can be effectively applied to real samples. This study provided a novel method for the non-invasive detection of the fecal antigen H. pylori.

Graphical abstract: Ultrasensitive detection of H. pylori in human feces based on immunomagnetic bead capture and fluorescent quantum dots

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Supplementary files

Article information


Submitted
26 Jan 2019
Accepted
29 Apr 2019
First published
09 May 2019

Analyst, 2019,144, 4086-4092
Article type
Paper

Ultrasensitive detection of H. pylori in human feces based on immunomagnetic bead capture and fluorescent quantum dots

L. Chen, X. Li, T. Zou, T. Wang, X. Cui, Y. Chen, C. Zhang and S. Zhao, Analyst, 2019, 144, 4086
DOI: 10.1039/C9AN00193J

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