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Issue 6, 2019
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Fluorescence lifetime imaging of intracellular magnesium content in live cells

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Abstract

The first detailed analysis of FLIM applications for Mg cell imaging is presented. We employed the Mg-sensitive fluorescent dye named DCHQ5, a derivative of diaza-18-crown-6 ethers appended with two 8-hydroxyquinoline groups, to perform fluorescence lifetime imaging in control and Mg deprived SaOS-2 live cells, which contain different concentrations of magnesium. We found that the lifetime maps are almost uniform all over the cells and, most relevantly, we showed that the ratio of the amplitude terms is related to the magnesium intracellular concentration.

Graphical abstract: Fluorescence lifetime imaging of intracellular magnesium content in live cells

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Publication details

The article was received on 07 Dec 2018, accepted on 18 Feb 2019 and first published on 19 Feb 2019


Article type: Communication
DOI: 10.1039/C8AN02379D
Analyst, 2019,144, 1876-1880

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    Fluorescence lifetime imaging of intracellular magnesium content in live cells

    A. Sargenti, A. Candeo, G. Farruggia, C. D'Andrea, C. Cappadone, E. Malucelli, G. Valentini, P. Taroni and S. Iotti, Analyst, 2019, 144, 1876
    DOI: 10.1039/C8AN02379D

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