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Issue 6, 2019
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Fluorescence lifetime imaging of intracellular magnesium content in live cells

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Abstract

The first detailed analysis of FLIM applications for Mg cell imaging is presented. We employed the Mg-sensitive fluorescent dye named DCHQ5, a derivative of diaza-18-crown-6 ethers appended with two 8-hydroxyquinoline groups, to perform fluorescence lifetime imaging in control and Mg deprived SaOS-2 live cells, which contain different concentrations of magnesium. We found that the lifetime maps are almost uniform all over the cells and, most relevantly, we showed that the ratio of the amplitude terms is related to the magnesium intracellular concentration.

Graphical abstract: Fluorescence lifetime imaging of intracellular magnesium content in live cells

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Supplementary files

Article information


Submitted
07 Dec 2018
Accepted
18 Feb 2019
First published
19 Feb 2019

Analyst, 2019,144, 1876-1880
Article type
Communication

Fluorescence lifetime imaging of intracellular magnesium content in live cells

A. Sargenti, A. Candeo, G. Farruggia, C. D'Andrea, C. Cappadone, E. Malucelli, G. Valentini, P. Taroni and S. Iotti, Analyst, 2019, 144, 1876
DOI: 10.1039/C8AN02379D

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