Jump to main content
Jump to site search

Issue 8, 2019
Previous Article Next Article

A paper-based chemiluminescence immunoassay device for rapid and high-throughput detection of allergen-specific IgE

Author affiliations

Abstract

The fast and precise detection of potential allergen-specific immunoglobulin E (sIgE) is imperative for the diagnosis and appropriate treatment of allergic diseases. In this study, we have successfully fabricated a novel paper-based immunoassay device for the detection of sIgE in allergic diseases. We used Can f 1, one of the main dog allergens, as a model allergen to detect sIgE in human sera. To achieve excellent performance, the experimental parameters were optimized. Further, we extended this device for potential applications in the clinical diagnosis of allergic diseases: worthwhile clinical performance in the detection of allergens was achieved as compared to that achieved by commercial enzyme-linked immunosorbent assay (ELISA) kit. Therefore, it was proven that this strategy has the advantages of high-throughput, rapid, sensitive, and highly accurate detection of trace amounts of sIgEs. Furthermore, by simply changing the antigen and antibody, this device could be used for the high-throughput detection of other allergens, so as to achieve multiallergen detection and appropriate desensitization therapy, thereby making it promising in the determination of allergic diseases in clinics.

Graphical abstract: A paper-based chemiluminescence immunoassay device for rapid and high-throughput detection of allergen-specific IgE

Back to tab navigation

Supplementary files

Article information


Submitted
21 Oct 2018
Accepted
13 Feb 2019
First published
18 Feb 2019

Analyst, 2019,144, 2584-2593
Article type
Paper

A paper-based chemiluminescence immunoassay device for rapid and high-throughput detection of allergen-specific IgE

X. Han, M. Cao, M. Wu, Y. Wang, C. Yu, C. Zhang, H. Yu, J. Wei, L. Li and W. Huang, Analyst, 2019, 144, 2584
DOI: 10.1039/C8AN02020E

Social activity

Search articles by author

Spotlight

Advertisements