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Issue 5, 2019
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Phosphoric acid and phosphorylation levels are potential biomarkers indicating developmental competence of matured oocytes

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Abstract

Here, we aimed to identify biomarkers for mice oocyte maturation in metaphase II in vivo and in situ using Raman spectroscopy. Principal component analysis of 324 Raman data points of oocytes at Phase I, II, III, and IV showed that the phosphoric acid concentration uniformly increased in oocytes with higher developmental competence than in oocytes at other maturation stages, and proteins were more phosphorylated. The maturation phases were successfully predicted by linear discriminant analysis with high accuracy (90.7%) using phosphoric molecular information mentioned above. Furthermore, detections of higher concentration of unsaturated fatty acids in overmatured oocytes indicated that a decline in metabolic activity due to overmaturation induced a surplus of these lipid components. Upon assessing invasiveness by laser irradiation, about 50% irradiated oocytes progressed to morula and blastocyst stages in good conditions. Thus, Raman spectroscopy holds promise in evaluating oocyte maturation and quality based on molecular information in infertility treatment.

Graphical abstract: Phosphoric acid and phosphorylation levels are potential biomarkers indicating developmental competence of matured oocytes

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Publication details

The article was received on 16 Aug 2018, accepted on 14 Jan 2019 and first published on 21 Jan 2019


Article type: Paper
DOI: 10.1039/C8AN01589A
Analyst, 2019,144, 1527-1534

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    Phosphoric acid and phosphorylation levels are potential biomarkers indicating developmental competence of matured oocytes

    M. Ishigaki, Y. Hoshino and Y. Ozaki, Analyst, 2019, 144, 1527
    DOI: 10.1039/C8AN01589A

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