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Issue 21, 2018
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A facile AuNPs@aptamer-modified mercaptosiloxane-based hybrid affinity monolith with an unusually high coverage density of aptamer for on-column selective extraction of ochratoxin A

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Abstract

A convenient and high-performance AuNPs@aptamer-modified mercaptosiloxane-based hybrid affinity monolithic column with an unusually high coverage density of aptamers was facilely prepared and used for on-column selective recognition of ochratoxin A (OTA). Due to the high surface-to-volume ratio of AuNPs, the robust conjugation of Au-SH and large specific surface area of hybrid-silica monolith, high coverage density of 5′-SH-aptamers up to 3494 pmol μL−1 was achieved, which was 2.5–10 folds higher than that of other previously reported affinity monoliths modified with AuNPs@Apt. Using OTA as the model analyte, the highly selective recognition of OTA was carried out via online coupling with HPLC, and the cross-reactivity towards analogues, such as OTB and aflatoxin B1, was weak. High recovery yields of OTA were achieved at more than 92% (n = 3) even when OTB was added at a high concentration level up to 50 ng mL−1. For sample analysis, efficient discrimination of OTA was successfully obtained with a sensitive detection limit of 25 pg mL−1. The recoveries of OTA with different fortified levels were achieved at 88.6%–94.1% and 88.2%–94.3% for beer and wine samples, respectively. This protocol provides a facile approach for fabricating a desirable affinity monolith modified with abundant aptamers for highly selective and sensitive on-column extraction of target analyte OTA.

Graphical abstract: A facile AuNPs@aptamer-modified mercaptosiloxane-based hybrid affinity monolith with an unusually high coverage density of aptamer for on-column selective extraction of ochratoxin A

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Supplementary files

Article information


Submitted
08 Aug 2018
Accepted
14 Sep 2018
First published
14 Sep 2018

Analyst, 2018,143, 5210-5217
Article type
Paper

A facile AuNPs@aptamer-modified mercaptosiloxane-based hybrid affinity monolith with an unusually high coverage density of aptamer for on-column selective extraction of ochratoxin A

J. Chi, M. Chen, L. Deng, X. Lin and Z. Xie, Analyst, 2018, 143, 5210
DOI: 10.1039/C8AN01531G

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