Jump to main content
Jump to site search

Issue 24, 2018
Previous Article Next Article

Raman spectroscopic features of primary cardiac microvascular endothelial cells (CMECs) isolated from the murine heart

Author affiliations

Abstract

Gaining knowledge on the biochemical profile of primary endothelial cells on a subcellular level can contribute to better understanding of cardiovascular disease. In this work, primary cardiac microvascular endothelial cells (CMECs) isolated from the mouse heart and murine H5V endothelial cell line were characterized with the use of a Raman imaging technique. Primary CMECs displayed a distinct Raman-based biochemical phenotype as compared with other cells isolated from the heart and were characterized by a low lipid content. In contrast to the murine H5V endothelial cell line, CMECs did not display lipid droplets (LDs) in the cytoplasm, while the former have many low-unsaturated LDs. In conclusion, Raman imaging is a fast and efficient tool to analyse single coronary endothelial cells in a non-invasive manner that can prove useful to characterize biochemical changes in a single isolated primary endothelial cell from a diseased heart.

Graphical abstract: Raman spectroscopic features of primary cardiac microvascular endothelial cells (CMECs) isolated from the murine heart

Back to tab navigation

Supplementary files

Article information


Submitted
15 Jul 2018
Accepted
01 Nov 2018
First published
05 Nov 2018

Analyst, 2018,143, 6079-6086
Article type
Paper

Raman spectroscopic features of primary cardiac microvascular endothelial cells (CMECs) isolated from the murine heart

S. Tott, M. Grosicki, B. Klimas, D. Augustynska, S. Chlopicki and M. Baranska, Analyst, 2018, 143, 6079 DOI: 10.1039/C8AN01308J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements