Issue 12, 2018

An enzymatic reaction mediated glucose sensor activated by MnO2 nanosheets acting as an oxidant and catalyst

Abstract

A self-regulated smart system would be highly desirable for analyte detection, in which a specific environment for detection could be self-modulated and the required reagents could also be in situ generated without further addition. Here, we have designed an intelligent glucose detection system composed of glucose, glucose oxidase (GOx), MnO2 and 3,3′,5,5′-tetramethylbenzidine (TMB), based on the enzymatic oxidation of glucose and dual roles of synthesized MnO2 nanosheets acting as both an oxidant and catalyst. Upon the addition of glucose/GOx, the MnO2 nanosheets partially decompose due to H2O2in situ generated via glucose oxidation. Following the addition of TMB, a typical color reaction occurs under slightly acidic conditions, thereby enabling the colorimetric determination of glucose. For this system, the specific conditions and the required reagents for glucose detection can be self-modulated and self-generated via the enzymatic oxidation of glucose to gluconic acid and H2O2, performing smart self-regulated functions. This is an outstanding advantage of our designed glucose sensing system. Moreover, the present sensing system responds to glucose quickly and sensitively with a detection limit of 100 nM, and is stable and specific toward glucose detection.

Graphical abstract: An enzymatic reaction mediated glucose sensor activated by MnO2 nanosheets acting as an oxidant and catalyst

Supplementary files

Article information

Article type
Paper
Submitted
10 Apr 2018
Accepted
30 Apr 2018
First published
01 May 2018

Analyst, 2018,143, 2915-2922

An enzymatic reaction mediated glucose sensor activated by MnO2 nanosheets acting as an oxidant and catalyst

G. Yan, Y. Zhang and W. Di, Analyst, 2018, 143, 2915 DOI: 10.1039/C8AN00657A

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