FT-IR- and Raman-based biochemical profiling of the early stage of pulmonary metastasis of breast cancer in mice

Karolina Chrabaszcz; Kamila Kochan; Andrzej Fedorowicz; Agnieszka Jasztal; Elzbieta Buczek; Lisa S. Leslie; Rohit Bhargava; Kamilla Malek; Stefan Chlopicki; Katarzyna M. Marzec

doi:10.1039/C7AN01883E

FT-IR- and Raman-based biochemical profiling of the early stage of pulmonary metastasis of breast cancer in mice†

Karolina Chrabaszcz,^abc Kamila Kochan,

^d Andrzej Fedorowicz,

^ea Agnieszka Jasztal,^a Elzbieta Buczek,^a Lisa S. Leslie,^f Rohit Bhargava,^fg Kamilla Malek,

^c Stefan Chlopicki^ae and Katarzyna M. Marzec

*^ab

Author affiliations

* Corresponding authors

^a Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, Krakow, Poland
E-mail: katarzyna.marzec@jcet.eu

^b Centre for Medical Genomics OMICRON, Jagiellonian University Medical College, Krakow, Poland

^c Faculty of Chemistry, Jagiellonian University, Krakow, Poland

^d Centre for Biospectroscopy, School of Chemistry, Monash University, Wellington Rd, Clayton, Vic., 3800 Australia

^e Chair of Pharmacology, Jagiellonian University Medical College, Krakow, Poland

^f Department of Bioengineering and Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana–Champaign, Urbana, USA

^g Department of Mechanical Science and Engineering, Electrical and Computer Engineering, Micro and Nanotechnology Laboratory and University of Illinois Cancer Center, University of Illinois at Urbana–Champaign, Urbana, USA

Abstract

The combination of FT-IR and Raman spectroscopies allowed the biochemical profiling of lungs in the early stage of pulmonary metastasis in the murine model of breast cancer. Histological staining was used as a reference. Raman spectroscopy was especially useful in the detection and semi-quantitative analysis of the vitamin A content in lung lipofibroblasts, whereas the IR technique provided semi-quantitative information on the contents of nucleic acids, carbohydrates including glycogen, and lipids as well as changes in the secondary structures of tissue proteins. Our spectroscopic results suggest that the early phase of metastasis in the lung is characterized by a decrease in the endogenous retinoid content in combination with a decrease in the content of glycogen and lipids.

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FT-IR- and Raman-based biochemical profiling of the early stage of pulmonary metastasis of breast cancer in mice†

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FT-IR- and Raman-based biochemical profiling of the early stage of pulmonary metastasis of breast cancer in mice

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