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Issue 9, 2018
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FT-IR- and Raman-based biochemical profiling of the early stage of pulmonary metastasis of breast cancer in mice

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Abstract

The combination of FT-IR and Raman spectroscopies allowed the biochemical profiling of lungs in the early stage of pulmonary metastasis in the murine model of breast cancer. Histological staining was used as a reference. Raman spectroscopy was especially useful in the detection and semi-quantitative analysis of the vitamin A content in lung lipofibroblasts, whereas the IR technique provided semi-quantitative information on the contents of nucleic acids, carbohydrates including glycogen, and lipids as well as changes in the secondary structures of tissue proteins. Our spectroscopic results suggest that the early phase of metastasis in the lung is characterized by a decrease in the endogenous retinoid content in combination with a decrease in the content of glycogen and lipids.

Graphical abstract: FT-IR- and Raman-based biochemical profiling of the early stage of pulmonary metastasis of breast cancer in mice

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Article information


Submitted
21 Nov 2017
Accepted
11 Mar 2018
First published
15 Mar 2018

Analyst, 2018,143, 2042-2050
Article type
Paper

FT-IR- and Raman-based biochemical profiling of the early stage of pulmonary metastasis of breast cancer in mice

K. Chrabaszcz, K. Kochan, A. Fedorowicz, A. Jasztal, E. Buczek, L. S. Leslie, R. Bhargava, K. Malek, S. Chlopicki and K. M. Marzec, Analyst, 2018, 143, 2042
DOI: 10.1039/C7AN01883E

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