Issue 3, 2018

Competitive annealing mediated isothermal amplification of nucleic acids

Abstract

A novel nucleic acid isothermal amplification method with high specificity, efficiency and rapidity was developed. The principle of primer design was achieved by slight modification of PCR primers. Acceleration probes were also explored. The newly developed method is of great potential for point of care diagnostics.

Graphical abstract: Competitive annealing mediated isothermal amplification of nucleic acids

Supplementary files

Article information

Article type
Communication
Submitted
21 Sep 2017
Accepted
16 Dec 2017
First published
19 Dec 2017

Analyst, 2018,143, 639-642

Competitive annealing mediated isothermal amplification of nucleic acids

R. Mao, L. Qi, J. Li, M. Sun, Z. Wang and Y. Du, Analyst, 2018, 143, 639 DOI: 10.1039/C7AN01569K

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