Issue 21, 2016

Designed miniaturization of microfluidic biosensor platforms using the stop-flow technique

Abstract

Here, we present a novel approach to increase the degree of miniaturization as well as the sensitivity of biosensor platforms by the optimization of microfluidic stop-flow techniques independent of the applied detection technique (e.g. electrochemical or optical). The readout of the labeled bioassays, immobilized in a microfluidic channel, under stop-flow conditions leads to a rectangular shaped peak signal. Data evaluation using the peak height allows for a high level miniaturization of the channel geometries. To study the main advantages and limitations of this method by numerical simulations, a universally applicable model system is introduced for the first time. Consequently, proof-of-principle experiments were successfully performed with standard and miniaturized versions of an electrochemical biosensor platform utilizing a repressor protein-based assay for tetracycline antibiotics. Herein, the measured current peak heights are the same despite the sextuple reduction of the channel dimensions. Thus, this results in a 22-fold signal amplification compared to the constant flow measurements in the case of the miniaturized version.

Graphical abstract: Designed miniaturization of microfluidic biosensor platforms using the stop-flow technique

Supplementary files

Article information

Article type
Paper
Submitted
12 Jun 2016
Accepted
15 Aug 2016
First published
15 Aug 2016
This article is Open Access
Creative Commons BY license

Analyst, 2016,141, 6073-6079

Designed miniaturization of microfluidic biosensor platforms using the stop-flow technique

C. Dincer, A. Kling, C. Chatelle, L. Armbrecht, J. Kieninger, W. Weber and G. A. Urban, Analyst, 2016, 141, 6073 DOI: 10.1039/C6AN01330A

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