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Issue 21, 2016
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Fluorescence detection of single-nucleotide differences using aptamer-forming binary DNA probes

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Abstract

We report a simple method for fluorescence detection of single-nucleotide alterations in a long target DNA, which is based on the formation of a three-way-junction-structured cholic-acid-binding DNA aptamer by the hybridization of the target with binary DNA probes. The new method was successfully exploited for SNP genotyping of human CYP2C19 gene.

Graphical abstract: Fluorescence detection of single-nucleotide differences using aptamer-forming binary DNA probes

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Publication details

The article was received on 19 Apr 2016, accepted on 07 Aug 2016 and first published on 08 Aug 2016


Article type: Paper
DOI: 10.1039/C6AN00912C
Analyst, 2016,141, 6087-6092

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    Fluorescence detection of single-nucleotide differences using aptamer-forming binary DNA probes

    S. Kamoto, M. Hyuga and T. Kato, Analyst, 2016, 141, 6087
    DOI: 10.1039/C6AN00912C

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