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Issue 23, 2016
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PCR free multiple ligase reactions and probe cleavages for the SNP detection of KRAS mutation with attomole sensitivity

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Abstract

A method to produce multiple ligated primers without PCR for a target DNA containing a single point mutation is presented. A strand displacing hairpin was introduced into the reaction, enabling separation of the ligated primer target DNA duplex without any thermal denaturing process. The ligated product was cycled to allow cleavage of fluorescence labeled substrates for RNase H on gold nanoparticles, leading to target specific fluorescence amplification. As a result, 10 attomoles of target DNA containing a point mutation in the KRAS gene were detected.

Graphical abstract: PCR free multiple ligase reactions and probe cleavages for the SNP detection of KRAS mutation with attomole sensitivity

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Publication details

The article was received on 19 Apr 2016, accepted on 16 Oct 2016 and first published on 19 Oct 2016


Article type: Communication
DOI: 10.1039/C6AN00909C
Analyst, 2016,141, 6381-6386

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    PCR free multiple ligase reactions and probe cleavages for the SNP detection of KRAS mutation with attomole sensitivity

    J. H. Kim, Analyst, 2016, 141, 6381
    DOI: 10.1039/C6AN00909C

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