Jump to main content
Jump to site search

Issue 18, 2016

The correction fluorescence inner filter effect using a single excitation and dual-emission fiber optic probe

Author affiliations

Abstract

A significant disadvantage of fluorometry is the inner filter effect when the fluorophore concentration is high. A new simple fiber-optic probe was made to measure the concentration of fluorescent solutions. The proposed probe consists of one excitation fiber and two emission fibers. One emission fiber is parallel to the excitation fiber, and the other has a tilted angle with the excitation fiber. A numerical model was used to optimize the tilted angle and distance of the three fibers. There was a linear relationship between the fluorophore concentration and the ratio of the fluorescence intensity of the two emission fibers. Using our homemade probe, we measured Eosin Y, fluorescein and quinine sulfate solutions. The linear range of Eosin Y solution was up to 500 μM, which was approximately 7 times the range measured with a single emission probe. The results of fluorescein and quinine sulfate solutions also showed that the fluorescence intensity ratio method could correct the inner filter effect. The experimental results also indicated that the probe is robust when the excitation light fluctuates.

Graphical abstract: The correction fluorescence inner filter effect using a single excitation and dual-emission fiber optic probe

Article information


Submitted
11 Apr 2016
Accepted
08 Jun 2016
First published
08 Jun 2016

Analyst, 2016,141, 5339-5345
Article type
Paper

The correction fluorescence inner filter effect using a single excitation and dual-emission fiber optic probe

L. Zeng, C. Wang, T. Wang and D. Li, Analyst, 2016, 141, 5339 DOI: 10.1039/C6AN00836D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements